Procyanidins From Japanese Quince (Chaenomeles Japonica) Fruit Induce Apoptosis in Human Colon Cancer Caco-2 Cells in a Degree of Polymerization-Dependent Manner
Menée sur des lignées cellulaires humaines et murines, cette étude montre que l'extrait de coing japonais, riche en procyanidines, peut induire l'apoptose des cellules cancéreuses du côlon
Plant proanthocyanidins, including procyanidins, display various biological activities. Here we report an inhibition of human colon cancer Caco-2 cell growth by the extract from Japanese quince fruit and the procyanidin-rich fractions of the extract. We observed that the amount of apoptotic Caco-2 cells increased by 52.1% vs. control after 72-h incubation with 50 ?g extract/mL, as assessed by flow cytometry and image cytometry. Under the same experimental conditions the corresponding values for human colon cancer HT-29 cells and for rat normal intestinal IEC-6 cells were 5.0% and 8.1%, respectively. The extract fractions enriched with higher oligomers exhibited the highest proapoptotic activity. In conclusion, the Japanese quince procyanidins exhibited proapoptotic activity in Caco-2 cells within a submilimolar concentration range.