Small nucleolar RNA expression profiling identifies potential prognostic markers in peripheral T-cell lymphoma
Menée sur une cohorte de 122 patients atteints d'un lymphome T périphérique, cette étude évalue l'association entre l'expression de 80 petits ARNs nucléolaires et le pronostic de la maladie
Peripheral T-cell Lymphomas (PTCL) are types of rare and heterogeneous Non-Hodgkin's Lymphoma (NHL) that, in general, are associated with a poor clinical outcome. Discovery of new prognostic tools is thus a current and major challenge. A cohort of 122 cases of PTCL was collected from a multicentric T-cell lymphoma consortium (TENOMIC). We analyzed the expression of 80 small nucleolar RNAs (snoRNAs) using high-throughput quantitative PCR (Fluidigm). We demonstrate that snoRNA expression analysis may be useful in both the diagnosis of some subtypes of PTCL and the prognostication of both PTCL-Not Otherwise Specified (PTCL-NOS) (n=26) and Angio-Immunoblastic T-cell Lymphoma (AITL) (n=46) patients treated with chemotherapy. Like microRNAs, snoRNAs were globally down-regulated in tumor cells compared to their normal counterparts. The snoRNA signature was robust enough to differentiate Anaplastic Large Cell Lymphoma (ALCL) (n=32) from other PTCLs. For PTCL-NOS and AITL we obtained two distinct prognostic signatures with a reduced set of 3 genes. Of particular interest was the prognostic value of HBII-239 snoRNA which was significantly over-expressed in cases of AITL and PTCL-NOS that had favourable outcomes. Overall, we demonstrate that snoRNA expression profiles may have a diagnostic and prognostic significance for PTCL, offering new tools for patient care and follow-up.
Blood , résumé, 2012