Enhanced anti-cancer activity of human dendritic cells sensitized with gamma-irradiation-induced apoptotic colon cancer cells
Menée sur une lignée cellulaire humaine de cancer du côlon, cette étude montre que l'irradiation des cellules dendritiques par rayonnements gamma améliore la réponse immunitaire antitumorale
Properly sensitized dendritic cells (DCs) can be an effective immunotherapeutic against cancers. We investigated the phenotypic and functional changes in human DCs sensitized with γ-irradiated colon cancer cell-line HT-29 (GIH). GIH induced maturation and activation of DCs. GIH-sensitized DCs showed increased cytotoxic activity against HT-29 through higher expression of perforin and granzyme B. They further induced expression of effector cytokines, cytotoxic molecules, and mucosal-homing receptor in autologous T-cells. Conclusively, these results suggest that effective anti-cancer activity is induced when DCs are sensitized with γ -irradiated cancer cells via both direct augmentation of the cytotoxicity and indirect activation of T cells.
http://www.sciencedirect.com/science/article/pii/S030438351300164X 2013