MicroRNA-365 Regulates NKX2-1, a Key Mediator of Lung Cancer
Menée in vitro, cette étude met en évidence un mécanisme par lequel, en régulant l'expression du gène NKX2-1, le micro-ARN 365 joue un rôle dans la prolifération des cellules de cancer du poumon
MicroRNAs constitute a class of small noncoding RNAs that play roles in tumorigenesis. We found that NKX2-1 protein levels were generally high in the lung cancer tissues whereas miRNA-365 expression levels were down-regulated. Ectopic miR-365 expression decreased NKX2-1 expression in lung cancer cell lines. Transfection of a miR-365 mimic led to reduced proliferation of lung cancer cells; conversely, a miR-365 inhibitor slightly increased cell proliferation. The NKX2-1 overexpression significantly increased the cell proliferation by overcoming the suppressive effect of miR-365. Our data suggest that miR-365 is an important regulator of NKX2-1 and can be a target for lung cancer therapies.
http://www.sciencedirect.com/science/article/pii/S0304383513002346