p53 Acts as a Safeguard of Translational Control by Regulating Fibrillarin and rRNA Methylation in Cancer
Menée in vitro, cette étude met en évidence des mécanismes par lesquels, en régulant l'expression de la fibrillarine et la méthylation de l'acide ribonucléique ribosomique, p53 assure la qualité des ribosomes
Ribosomes are specialized entities that participate in regulation of gene expression through their rRNAs carrying ribozyme activity. Ribosome biogenesis is overactivated in p53-inactivated cancer cells, although involvement of p53 on ribosome quality is unknown. Here, we show that p53 represses expression of the rRNA methyl-transferase fibrillarin (FBL) by binding directly to FBL. High levels of FBL are accompanied by modifications of the rRNA methylation pattern, impairment of translational fidelity, and an increase of internal ribosome entry site (IRES)-dependent translation initiation of key cancer genes. FBL overexpression contributes to tumorigenesis and is associated with poor survival in patients with breast cancer. Thus, p53 acts as a safeguard of protein synthesis by regulating FBL and the subsequent quality and intrinsic activity of ribosomes. "p53 directly represses transcription of the rRNA methyl-transferase fibrillarin "rRNA methylation pattern is altered in response to p53 inactivation "p53 inactivation promotes IRES-dependent translation in an FBL-dependent manner "High FBL mRNA levels are a marker of poor prognosis in breast cancer