Universal Marker and Detection Tool for Human Sarcoma Circulating Tumor Cells
Mené sur des lignées cellulaires, à l'aide de modèles murins et canins, ainsi que sur des échantillons sanguins prélevés sur des patients atteints d'un sarcome, cette étude identifie un biomarqueur, la vimentine, permettant de détecter et de dénombrer les cellules tumorales circulantes, quel que soit le tissu d'origine du sarcome
To date, no specific marker exists for the detection of circulating tumor cell from different types of sarcomas, though tools are available for detection of circulating tumor cell (CTC) in peripheral blood of cancer patients for epithelial cancers. Here we report cell-surface vimentin (CSV) as an exclusive marker on sarcoma CTC regardless of the tissue origin of the sarcoma as detected by a novel monoclonal antibody. Utilizing CSV as a probe we isolated and enumerated sarcoma CTC with high sensitivity and specificity from the blood of patients bearing different types of sarcoma, validating their phenotype by single cell genomic amplification, mutation detection and fluorescence in situ hybridization. Our results establish the first universal and specific CTC marker described for enumerating CTC from different types of sarcoma, thereby providing a key prognosis tool to monitor cancer metastasis and relapse.
Cancer Research , résumé, 2014