Hydroxytyrosol, a natural molecule from olive oil, suppresses the growth of human hepatocellular carcinoma cells via inactivating AKT and nuclear factor-kappa B pathways
Menée in vitro et à l'aide d'un modèle orthotopique de carcinome hépatocellulaire humain, cette étude met en évidence un mécanisme par lequel l'hydroxytyrosol, un composé polyphénolique présent dans l'huile d'olive, inhibe la croissance tumorale en inactivant les voies de la protéine kinase B et du facteur nucléaire kappa B
Scope : Hydroxytyrosol (HT), a natural polyphenolic compound in olive oil, has been proved to possess significant antitumor activity in several cancer cells. However, effects of HT on human hepatocellular carcinoma (HCC) cells are still largely unknown. The present study aimed to investigate the anti-cancer effects of HT in human HCC cells. Methods and results : We observed that HT could inhibit proliferation, induce G2/M cell cycle arrest and apoptosis in human HCC (HepG2, Hep3B, SK-HEP-1 and Huh7) cells in a dose-dependent manner. Mechanically, we found that HT could suppress the activation of AKT and nuclear factor-kappa B (NF-
κB) pathways, and the latter correlated with the suppression of NF-κB regulated gene products, including c-myc, cyclinD1, Bcl-2, Bcl-xL, COX-2 and VEGF. Most importantly, we show for the first time that HT also significantly inhibited the tumor growth, angiogenesis and the activation of AKT and NF-κB pathways in an orthotopic model of human HCC in vivo. Conclusion
:
HT could suppress the growth of HCC in vitro and in vivo, the mechanism is at least partially due to the suppressive effect of HT on the activation of AKT and NF-κB pathways. Thus it may be a promising candidate agent for the treatment of HCC.
http://www.sciencedirect.com/science/article/pii/S0304383514000664