Radiosensitization of tumor cells through endoplasmic reticulum stress induced by PEGylated nanogel containing gold nanoparticles
Menée in vitro, cette étude met en évidence un mécanisme par lequel un nanogel de polyéthylène glycol contenant des nanoparticules d'or sensibilise les cellules tumorales aux rayons X en favorisant la synthèse de protéines de stress au niveau du réticulum endoplasmique
High atomic number molecules, such as gold and platinum, are known to enhance the biological effect of X-irradiation. This study was aimed to determine the radiosensitizing potential of PEGylated nanogel containing gold nanoparticles (GNG) and the cellular mechanism involved. GNG pretreatment increased the levels of reproductive cell death and apoptosis induced by X-irradiation. GNG accumulated in cytoplasm and increased the expression of endoplasmic reticulum (ER) stress-related protein. GNG suppressed the repair capacity of DNA after X-irradiation by down-regulating DNA repair-related proteins. Our results suggest that GNG radiosensitized cells by enhancing apoptosis and impairing DNA repair capacity via ER stress induction.
http://www.sciencedirect.com/science/article/pii/S0304383514000962