PIK3R3 induces epithelial-to-mesenchymal transition and promotes metastasis in colorectal cancer
Menée in vitro et in vivo, cette étude met en évidence des mécanismes par lesquels la protéine PIK3R3 favorise le processus métastatique d'un cancer colorectal
Class IA phosphatidylinositol 3-kinase (PI3K) plays an essential role in the invasion and metastasis of cancer. However, the mechanisms and specific functions of PI3K isoforms in tumor invasion and metastasis are not fully understood. We evaluated the role of PIK3R3, a PI3K regulatory subunit encoded by the PIK3R3 gene, in colorectal cancer (CRC) invasion and metastasis. Clinic specimens and cell lines data shows the expression level of PIK3R3 is associated with CRC metastasis. Over-expression of PIK3R3 increases tumor migration and invasion in vitro, and promotes metastasis of CRCs in vivo. Furthermore, we investigates that over-expression of PIK3R3 depend on SNAI2 inducing significant epithelial-to-mesenchymal transition (EMT). Down-regulation of PIK3R3 reverses this process, which possibly contributes to the enhanced invasive and metastasizing abilities of CRC cells. In this study, we found that PIK3R3 plays an important role in CRC metastasis, and might be a potential and specific target for therapies against metastatic CRC.
http://mct.aacrjournals.org/content/early/2014/05/16/1535-7163.MCT-14-0049.abstract 2014