Modeling Lung Cancer Evolution and Pre-Clinical Response By Orthotopic Mouse Allografts
Cette étude évalue l'intérêt d'utiliser des allogreffes sur un modèle murin pour étudier l'évolution d'un cancer du poumon non à petites cellules et pour développer de nouvelles approches thérapeutiques
Cancer evolution is a process that is still poorly understood due to the lack of versatile in vivo longitudinal studies. By generating murine Non-Small Cell Lung Cancer (NSCLC) orthoallobanks and paired primary cell lines, we provide a detailed description of an in vivo, time-dependent cancer malignization process. We identify the acquisition of metastatic dissemination potential, the selection of co-driver mutations and the appearance of naturally occurring intra-tumor heterogeneity, thus recapitulating the stochastic nature of human cancer development. This approach combines the robustness of genetically engineered cancer models with the flexibility of allograft methodology. We have applied this tool for the pre-clinical evaluation of therapeutic approaches. This system can be implemented to improve the design of future treatments for NSCLC patients.
Cancer Research , résumé, 2014