Polygenic in vivo validation of cancer mutations using transposons
Cet article présente une méthode qui, à l'aide de transposons, permet de créer des modèles murins pour mener des études de validation de plusieurs gènes mutés associés au cancer
The in vivo validation of cancer mutations and genes identified in cancer genomics is resource-intensive because of the low throughput of animal experiments. We describe a mouse model that allows multiple cancer mutations to be validated in each animal line. Animal lines are generated with multiple candidate cancer mutations using transposons. The candidate cancer genes are tagged and randomly expressed in somatic cells, allowing easy identification of the cancer genes involved in the generated tumors. This system presents a useful, generalized and efficient means for animal validation of cancer genes.
Genome Biology , article en libre accès, 2013