Near infrared photoimmunotherapy in a transgenic mouse model of spontaneous epidermal growth factor receptor (EGFR)-expressing lung cancer
Menée à l'aide d'un modèle murin transgénique de cancer sporadique du poumon EGFR+, cette étude évalue l'effet d'une immunophotothérapie en proche infrarouge sur le volume tumoral mesuré par IRM
Near infrared photoimmunotherapy (NIR-PIT) is a new cancer treatment that combines the specificity of antibodies for targeting tumors with the toxicity induced by a sensitive photoabsorber following exposure to NIR light. Most studies of NIR-PIT have been performed in xenograft models of cancer. The purpose of this study was to evaluate the therapeutic effects of NIR-PIT in a transgenic model of spontaneous lung cancer expressing human epidermal growth factor receptor (hEGFR). Mice were separated into 3 groups for the following treatments: (1) no treatment (control); (2) 150
μg of photoabsorber, IR700, conjugated to panitumumab, an antibody targeting EGFR (antibody-photoabsorber conjugate (APC)) i.v. only; (3) 150 μg of APC i.v. with NIR light administration. Each treatment was performed every week up to three weeks. MRI was performed 1 day before and 3, 6, 13, 20, 27, and 34 days after first NIR-PIT. The relative volume of lung tumors was calculated from the tumor volume at each MRI time point divided by the initial volume. Steel's test for multiple comparison was used to compare the tumor volume ratio with that of control. Tumor volume ratio was inhibited significantly in the NIR-PIT group compared with control group (p < 0.01 at all time points). In conclusion, NIR-PIT effectively treated a spontaneous lung cancer in a hEGFR TL transgenic mouse model. MRI successfully monitored the therapeutic effects of NIR-PIT.
http://mct.aacrjournals.org/content/early/2016/11/15/1535-7163.MCT-16-0663.abstract