Mapping the in vivo fitness landscape of lung adenocarcinoma tumor suppression in mice
Menée notamment à l'aide de la technologie CRISPR/Cas9 et de modèles murins d'adénocarcinome du poumon, cette étude analyse les effets produits par des anomalies de gènes suppresseurs de tumeurs
The functional impact of most genomic alterations found in cancer, alone or in combination, remains largely unknown. Here we integrate tumor barcoding, CRISPR/Cas9-mediated genome editing and ultra-deep barcode sequencing to interrogate pairwise combinations of tumor suppressor alterations in autochthonous mouse models of human lung adenocarcinoma. We map the tumor suppressive effects of 31 common lung adenocarcinoma genotypes and identify a landscape of context dependence and differential effect strengths.
Nature Genetics 2018