Lactate concentration in breast cancer using advanced magnetic resonance spectroscopy
Menée à partir de tumeurs mammaires prélevées sur 30 patientes atteintes d'un carcinome invasif du sein, cette étude analyse la corrélation entre la concentration intratumorale de lactate, mesurée à l'aide d'une technique de spectroscopie par résonance magnétique, et le système de score "Nottingham Prognostic Index" ainsi que le niveau d'expression de la lactate déshydrogénase A et de l'antigène Ki-67
Background : Precision medicine in breast cancer demands markers sensitive to early treatment response. Aerobic glycolysis (AG) upregulates lactate dehydrogenase A (LDH-A) with elevated lactate production; however, existing approaches for lactate quantification are either invasive or impractical clinically.
Methods : Thirty female patients (age 39–78 years, 15 grade II and 15 grade III) with invasive ductal carcinoma were enrolled. Lactate concentration was quantified from freshly excised whole tumours with double quantum filtered (DQF) magnetic resonance spectroscopy (MRS), and Nottingham Prognostic Index (NPI), LDH-A and proliferative marker Ki-67 were assessed histologically.
Results : There was a significantly higher lactate concentration (t = 2.2224, p = 0.0349) in grade III (7.7 ± 2.9 mM) than in grade II (5.5 ± 2.4 mM). Lactate concentration was correlated with NPI (
ρ
= 0.3618, p = 0.0495), but not with Ki-67 (
ρ
= 0.3041, p = 0.1023) or tumour size (r = 0.1716, p = 0.3645). Lactate concentration was negatively correlated with LDH-A (
ρ
=
−
0.3734, p = 0.0421).
Conclusion : Our results showed that lactate concentration in whole breast tumour from DQF MRS is sensitive to tumour grades and patient prognosis.
British Journal of Cancer , résumé, 2020