TLR4 regulatory region variants reduce the susceptibility of small-cell lung cancer in Chinese population
Menée en Chine à l'aide du génotypage de 304 témoins et 304 patients atteints d'un cancer du poumon à petites cellules, cette étude analyse le rôle des variants des gènes des TLR dans la carcinogenèse pulmonaire
Objectives: Toll-like receptors (TLRs) participate in the induction and regulation of immune responses and are closely related to the occurrence and development of small-cell lung cancer (SCLC). This study aimed to investigate the impact of polymorphisms in the regulatory regions of TLRs on the susceptibility of SCLC. Methods: The case–control study included 304 SCLC patients and 304 healthy controls. TLRs gene polymorphisms were genotyped by PCR-restrictive fragment length polymorphism analysis and TaqMan assay. Unconditional logistic regression was used to estimate odds ratio (OR) and its 95% confidence interval (95% CI). Results: Our results showed that TLR4 rs1927914 GG genotype and TLR4 rs7869402 TT genotype reduced the risk of SCLC with OR (95% CI) of 0.54 (0.32–0.90) and 0.47 (0.28–0.80), respectively. Stratified analysis suggested that TLR4 rs1927914 GG genotypes significantly reduced the risk of SCLC among male (OR = 0.35; 95% CI, 0.18–0.69; P < 0.01), the younger patients (OR = 0.49; 95% CI, 0.26–0.94; P = 0.03) and non-drinkers (OR = 0.47; 95% CI, 0.24–0.89; P = 0.02). TLR4 rs7869402 CT or TT genotype significantly reduced the susceptibility to SCLC among male patients (OR = 0.37; 95% CI, 0.19–0.71, P < 0.01), the younger patients (OR = 0.41; 95% CI, 0.22–0.79; P < 0.01), smokers (OR = 0.25; 95% CI, 0.10–0.60; P < 0.01) and drinkers (OR = 0.31; 95% CI, 0.11–0.88; P = 0.03). TLR3 rs5743303, TLR4 rs11536891, TLR5 rs1640816 and TLR7 rs3853839 had no significant correlation with the risk of SCLC. Conclusions: These findings emphasized the important role of TLR4 in the development of SCLC.