Non-Invasive Detection of Microsatellite Instability in patients with Endometrial Cancer
Menée à partir de l'analyse par PCR numérique en gouttelettes (ddPCR) de prélèvements utérins obtenus par aspiration, d'échantillons tumoraux inclus en paraffine et d'échantillons plasmatiques provenant de 90 patientes atteintes d'un cancer de l'endomètre, cette étude évalue, en fonction de la nature des échantillons biologiques utilisés, la performance de la ddPCR pour détecter une instabilité des microsatellites
The analysis of mismatch repair proteins in solid tissue is the standard of care (SoC) for the microsatellite instability (MSI) characterization in endometrial cancer (EC). Uterine aspirates (UAs) or circulating-DNA (cfDNA) samples capture the intratumor heterogeneity and provide a more comprehensive and dynamic molecular diagnosis. Thus, MSI analysis by droplet-digital PCR (ddPCR) in UAs and cfDNA can provide a reliable tool to characterise and follow-up the disease. The UAs, paraffin-embedded tumour tissue (FFPE) and longitudinal plasma samples from a cohort of 90 EC patients were analysed using ddPCR panel and compared to the SoC. A high concordance (96.67%) was obtained between the analysis of MSI markers in UAs and the SoC. Three discordant cases were validated as unstable by ddPCR on FFPE samples. Besides, a good overall concordance (70.27%) was obtained when comparing the performance of the ddPCR assay on UAs and cfDNA in high-risk tumours. Importantly, our results also evidenced the value of MSI analysis to monitor the disease evolution. MSI evaluation in minimally invasive samples shows great accuracy and sensitivity and provides a valuable tool for the molecular characterization and follow-up of endometrial tumours, opening new opportunities for personalized management of EC. This article is protected by copyright. All rights reserved.
International Journal of Cancer , résumé, 2023