Cellular states associated with metastatic organotropism and survival in patients with pancreatic ductal adenocarcinoma
Menée à l'aide de xénogreffes, du séquençage de l'ARN de cellules cancéreuses et de données portant sur 744 patients atteints d'un adénocarcinome canalaire du pancréas traité par résection, cette étude met en évidence une association entre le profil transcriptionnel des cellules tumorales primitives, la localisation des métastases et la survie des patients
Most patients with localized pancreatic ductal adenocarcinoma (PDAC) experience recurrence after resection. Analysis of 744 patients with resected PDAC revealed that patients with initial isolated liver-metastatic recurrence (n = 100) had significantly worse overall survival than those with initial isolated lung-metastatic recurrence (n = 31). Using single-nucleus RNA sequencing in a representative cohort, we found that transcriptional profiles of primary cancer cells with liver-metastatic recurrence and lung-metastatic recurrence were correlated with those of normal liver and lung parenchymal cells, respectively, suggesting adoption of organ-specific metastatic programs at the primary site. These signatures were confirmed in transcriptomes of PDAC lung and liver metastases, primary lung and liver tumors, and organotropic PDAC xenograft models. These signatures were independent of large genomic events, and analysis of large-scale tumor profiling data showed no genetic alterations predictive of recurrence patterns. Additional analyses suggested that metastatic recurrence may be determined early in tumorigenesis and influenced by tumor-infiltrating immune cells. Thus, pre-existing cellular states within primary tumors appear to guide organ-specific metastatic relapse.
Nature Genetics , résumé, 2025